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Yarrowia lipolytica as a Metabolic Engineering Platform for the Production of Very-Long-Chain Wax Esters
journal contribution
posted on 2020-09-19, 17:14 authored by Qi Gao, Jing-Lin Yang, Xin-Ru Zhao, Shun-Cheng Liu, Zhi-Jie Liu, Liu-Jing Wei, Qiang HuaThe
oleaginous yeast Yarrowia lipolytica is an attractive cell factory platform strain and can be used for
sustainable production of high-value oleochemical products. Wax esters
(WEs) have a good lubricating property and are usually used as a base
for the production of advanced lubricants and emollient oils. In this
study, we reported the metabolic engineering of Y.
lipolytica to heterologously biosynthesize high-content
very-long-chain fatty acids (VLCFAs) and fatty alcohols and efficiently
esterify them to obtain very-long-chain WEs. Co-expression of fatty
acid elongases from different sources in Y. lipolytica could yield VLCFAs with carbon chain lengths up to 24. Combining
with optimization of the central metabolic modules could further enhance
the biosynthesis of VLCFAs. Furthermore, through the screening of
heterologous fatty acyl reductases (FARs), we enabled high-level production
of fatty alcohols. Genomic integration and heterologous expression
of wax synthase (WS) and FAR in a VLCFA-producing Y.
lipolytica strain yielded 95–650 mg/L WEs with
carbon chain lengths from 32 to 44. Scaled-up fermentation in 5 L
laboratory bioreactors significantly increased the production of WEs
to 2.0 g/L, the highest content so far in yeasts. This study contributes
to the further efficient biosynthesis of VLCFAs and their derivatives.
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Wax estersWSemollient oilsacid elongasesVLCFA-producing Ylipolytica strainoleochemical productsheterologous expressionMetabolic Engineering Platformwax synthaseoleaginous yeast Yarrowia lipolyticaheterologously biosynthesize high-c...Yarrowia lipolyticaacyl reductasescarbon chain lengthsGenomic integration5 L laboratory bioreactorsFARVery-Long-Chain Wax Esters44. Scaled-upcell factory platform strainlubricating propertyvery-long-chain WEs
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