Water-Soluble Chlorophyll Protein (WSCP) Stably Binds Two or Four Chlorophylls
journal contributionposted on 02.03.2017, 00:00 by Daniel M. Palm, Alessandro Agostini, Stefan Tenzer, Barbara M. Gloeckle, Mara Werwie, Donatella Carbonera, Harald Paulsen
Water-soluble chlorophyll proteins (WSCPs) of class IIa from Brassicaceae form tetrameric complexes containing one chlorophyll (Chl) per apoprotein but no carotenoids. The complexes are remarkably stable toward dissociation and protein denaturation even at 100 °C and extreme pH values, and the Chls are partially protected against photooxidation. There are several hypotheses that explain the biological role of WSCPs, one of them proposing that they function as a scavenger of Chls set free upon plant senescence or pathogen attack. The biochemical properties of WSCP described in this paper are consistent with the protein acting as an efficient and flexible Chl scavenger. At limiting Chl concentrations, the recombinant WSCP apoprotein binds substoichiometric amounts of Chl (two Chls per tetramer) to form complexes that are as stable toward thermal dissociation, denaturation, and photodamage as the fully pigmented ones. If more Chl is added, these two-Chl complexes can bind another two Chls to reach the fully pigmented state. The protection of WSCP Chls against photodamage has been attributed to the apoprotein serving as a diffusion barrier for oxygen, preventing its access to triplet excited Chls and, thus, the formation of singlet oxygen. By contrast, the sequential binding of Chls by WSCP suggests a partially open or at least flexible structure, raising the question of how WSCP photoprotects its Chls without the help of carotenoids.
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sequential bindingsubstoichiometric amountsform complexeschlorophyll proteinsWSCP photoprotectsclass IIaWSCP apoproteinplant senescenceprotein denaturationdiffusion barrierpH valuesBrassicaceae form tetrameric complexesWSCP ChlsWater-Soluble Chlorophyll ProteinChl scavengerpathogen attackChl concentrationsStably Bindssinglet oxygentwo-Chl complexes