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Download fileVolcano-Shaped Scanning Probe Microscopy Probe for Combined Force-Electrogram Recordings from Excitable Cells
journal contribution
posted on 2020-05-27, 13:36 authored by B. X. E. Desbiolles, M. T. M Hannebelle, E. de Coulon, A. Bertsch, S. Rohr, G. E. Fantner, P. RenaudAtomic force microscopy
based approaches have led to remarkable
advances in the field of mechanobiology. However, linking the mechanical
cues to biological responses requires complementary techniques capable
of recording these physiological characteristics. In this study, we
present an instrument for combined optical, force, and electrical
measurements based on a novel type of scanning probe microscopy cantilever
composed of a protruding volcano-shaped nanopatterned microelectrode
(nanovolcano probe) at the tip of a suspended microcantilever. This
probe enables simultaneous force and electrical recordings from single
cells. Successful impedance measurements on mechanically stimulated
neonatal rat cardiomyocytes in situ were achieved
using these nanovolcano probes. Furthermore, proof of concept experiments
demonstrated that extracellular field potentials (electrogram) together
with contraction displacement curves could simultaneously be recorded.
These features render the nanovolcano probe especially suited for
mechanobiological studies aiming at linking mechanical stimuli to
electrophysiological responses of single cells.