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Vibrational Sensing Using Infrared Nanoantennas: Toward the Noninvasive Quantitation of Physiological Levels of Glucose and Fructose
journal contribution
posted on 2019-07-05, 00:00 authored by Lucca Kühner, Rostyslav Semenyshyn, Mario Hentschel, Frank Neubrech, Cristina Tarín, Harald GiessenMonosaccharides, which include the
simple sugars such as glucose
and fructose, are among the most important carbohydrates in the human
diet. Certain chronic diseases, e.g., diabetes mellitus, are associated
with anomalous glucose blood levels. Detecting and measuring the levels
of monosaccharides in vivo or in aqueous solutions is thus of the
utmost importance in life science, health, and point-of-care applications.
Noninvasive sensing would avoid problems such as pain and potential
infection hazards. Here, with the help of surface enhanced infrared
absorption (SEIRA) spectroscopy, we demonstrate the reliable optical
detection in the mid-infrared spectral range of pure glucose and fructose
solutions as well as mixtures of both in aqueous solution. We utilize
a reflection flow cell geometry with physiologically relevant concentrations
as small as 10 g/L. As significant improvement over the standard baseline
correction employed in SEIRA applications, we utilize principal component
analysis (PCA) as machine learning algorithm, which is ideally suited
for the extraction of vibrational data. We anticipate our results
as important step in biosensing applications that will stimulate efforts
to further improve the employed SEIRA substrates, the noise level
of the spectroscopic light source, as well as the flow cell environment
en route to significantly higher sensitivities and quantitative analysis,
even in tear drops.
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Keywords
noise levelpoint-of-care applicationsPhysiological LevelsInfrared Nanoantennasbaseline correctionbiosensing applicationsglucose blood levelsPCASEIRA substrateslife sciencecomponent analysisNoninvasive Quantitationinfection hazardsreflection flow cell geometryvibrational dataFructose Monosaccharidesflow cell environmentfructose solutionsspectroscopic light sourceSEIRA applications