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Versatile Strategy for Oligonucleotide Derivatization. Introduction of Lanthanide(III) Chelates to Oligonucleotides

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journal contribution
posted on 07.07.2001, 00:00 by Jari Hovinen, Harri Hakala
Novel nucleosidic phosphoramidite blocks were synthesized by a Mitsunobu reaction between 2‘-deoxy-5‘-O-(4,4‘-dimethoxytrityl)uridine and a primary alcohol containing a conjugate group in its structure (a protected functional group, an organic dye, or a precursor of a lanthanide(III) chelate) followed by phosphitylation. They were used in machine-assisted DNA synthesis in the standard manner. A slighty modified deprotection procedure was used for the preparation of oligonucleotide conjugates tethered to lanthanide(III) chelates. For the latter application one non-nucleosidic block was also synthesized.