Verification of the Role
of MlrC in Microcystin Biodegradation
by Studies Using a Heterologously Expressed Enzyme

Dziga, Dariusz; Wasylewski, Marcin; Szetela, Adam; Bocheńska, Oliwia; Wladyka, Benedykt

doi:10.1021/tx300174e.s001

tx300174e_si_001.pdf (130.5 kB)

Verification of the Role of MlrC in Microcystin Biodegradation by Studies Using a Heterologously Expressed Enzyme

journal contribution

posted on 2012-06-18, 00:00 authored by Dariusz Dziga, Marcin Wasylewski, Adam Szetela, Oliwia Bocheńska, Benedykt Wladyka

The MlrC protein from Sphingomonas ACM-3962 strain was heterologously expressed in Escherichia coli strain BL21(DE3) and purified to investigate participation of this enzyme in the biodegradation of two microcystin variants. In contrast with previous reports, our results indicated that MlrC cleaves linear microcystins, thus shedding new light on the role of MlrC enzyme in microcystin biodegradation.