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Download fileUse of Solution-IEF-Fractionation Leads to Separation of 2673 Mouse Brain Proteins Including 255 Hydrophobic Structures
journal contribution
posted on 2006-05-05, 00:00 authored by Jae-Kyung Myung, Gert LubecAnalyzing complex protein mixtures on a single
gel does not allow separation of many extracted proteins.
Herein, we tried a prefractionation approach and mouse
brain proteins were separated on a narrow pH range
ZOOM-IEF Fractionator (MicroSol-IEF device) and run on
two-dimensional gel electrophoresis. A total number of
2673 protein spots including 255 hydrophobic structures
were successfully analyzed by mass spectrometry. This
nonsophisticated approach to increase protein identification of a brain protein extract is a step forward in
neurochemistry.
Keywords: mouse brain • prefractionation • MicroSol-IEF •
solution isoelectricfocusing • mass spectrometry • MALDI • two-dimensional gel electrophoresis