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Download fileUpconversion Nanoprobes for the Ratiometric Luminescent Sensing of Nitric Oxide
journal contribution
posted on 2017-08-27, 00:00 authored by Ningning Wang, Xinyan Yu, Ke Zhang, Chad A. Mirkin, Jishan LiBy
taking advantage of the optical properties of upconversion nanoparticles
(UCNPs), we have designed a luminescence ratiometric nanosensor for
measuring nitric oxide (NO) in biological fluids, live cells, and
tissues. This nanoconjugate consists of a UCNP core with two strong
fluorescence emission peaks at 540 and 656 nm as the upconversion
fluorophore, NO-reactive rhodamine B-derived molecules (RdMs) encapsulated
within the mesopores of the mSiO2 shell, and a β-cyclodextrin
(βCD) layer on the exterior of the particle. Reaction of the
analyte with the O-phenylenediamine of the RdM induces
opening of the spiro-ring and is accompanied by an appearance of a
strong rhodamine B (RdB) absorption band between 500 and 600 nm, which
has spectral overlap with the green emission (540 nm) of the UCNPs.
This results in an increase in the I656/I540 ratio and quantitatively correlates
with [NO]. The assay is validated under clean buffer conditions as
well as inserum and liver tissue slices obtained from mouse models.
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600 nm540 ratiomouse modelsNO-reactive rhodamine B-derived molecules656 nmRdMupconversion fluorophoreUCNP coreluminescence ratiometric nanosensorfluorescence emission peaksNitric OxideRatiometric Luminescentabsorption bandrhodamine BUpconversion NanoprobesmSiO 2 shellliver tissue slicesnitric oxideupconversion nanoparticlesbuffer conditions