posted on 2021-12-25, 20:29authored byYinjun Tang, Yu Wu, Weiqing Xu, Lei Jiao, Yifeng Chen, Meng Sha, Hua-Rong Ye, Wenling Gu, Chengzhou Zhu
Noble-metal-based
nanozymes have attracted great interest as enzyme
mimics because of their unique properties. To modulate the performance
and meet the requirements of practical biosensing applications, phase
engineering is promising for the design of novel noble-metal-based
nanomaterials. Herein, a simple salt-assist strategy was employed
for the synthesis of Ru nanosheets (NSs) with the controlled crystalline
degree. The crystalline degree plays a significant role in tuning
peroxidase-like activity by optimizing the affinity toward the catalytic
substrate. Furthermore, the inhibition effect of mercapto molecules
on the peroxidase-like activity of Ru NSs was investigated. As a proof-of-concept,
the Ru NSs-based colorimetric sensing arrays were developed to distinguish
mercapto molecules, and five model molecules were well classified
according to the different inhibition effects. Given the complexity
of practical conditions, the sensing array was further applied to
discriminate proteins possessing rich mercapto groups. This work not
only provides an effective strategy for the design of highly active
nanozymes but also achieves promising sensing arrays for practical
needs.