posted on 2020-03-09, 18:36authored byLing-Hong Xiong, Suibin Huang, Yalan Huang, Feng Yin, Fan Yang, Qian Zhang, Jinquan Cheng, Renli Zhang, Xuewen He
Sensitive
and accurate diagnosis of viral infection is important for human health
and social safety. Herein, by means of explosive catalysis from an
enzyme muster, a powerful naked-eye readout platform has been successfully
constructed for ultrasensitive immunoassay of viral entities. Liposomes
were used to encapsulate multiple enzymes into an active unit. In
addition, its triggered rupture could boost the disassembly of gold
nano-aggregates that were cross-linked by peptides with opposite charges.
As a result, plasmonically colorimetric signals were rapidly generated
for naked-eye observation. Further harnessing the immunocapture, enterovirus
71 (EV71), a class of highly infective virus, was sensitively assayed
with a detection limit down to 16 copies/μL. It is superior
to the single enzyme-anchored immunoassay system. Most importantly,
the colorimetric assay was demonstrated with 100% clinical accuracy,
displaying strong anti-interference capability. It is expectable that
this sensitive, accurate, and convenient strategy could provide a
prospective alternative for viral infection analysis, especially in
resource-constrained settings.