posted on 2023-07-21, 17:37authored byJingjing Zhang, Fenglian Guo, Jingfeng Zhu, Zhimei He, Lin Hao, Lixing Weng, Lianhui Wang, Jie Chao
The development of noninvasive and sensitive detection
methods
for the early diagnosis and monitoring of bladder cancer is critical
but challenging. Herein, an ultrasensitive electrochemiluminescence
(ECL) immunosensor that uses Ru(bpy)32+–metal–organic
framework (Ru-MOF) nanospheres and a DNA tetrahedral (TDN) probe was
established for bladder cancer marker complement factor H-related
protein (CFHR1) detection. The synthesized Ru(bpy)32+–metal–organic frameworks (Ru-MOFs) served
as a linked substrate for immobilization of AuNPs and antibody (Ab2) to prepare the ECL signal probe (Ru-MOF@AuNPs-Ab2), exhibiting a stable and strengthened ECL emission. At the same
time, the inherent advantages of TDN probes on the electrode as the
capture probe (TDN-Ab1) improve the accessibility of targets
to probes. In the presence of CFHR1, the signal probe Ru-MOF@AuNPs-Ab2 was modified on the electrode through immune binding, thereby
obtaining an outstanding ECL signal. As expected, the developed ECL
immunosensor exhibited splendid performance for CFHR1 detection in
the range of 0.1 fg/mL to 10 pg/mL with a quite low detection limit
of 0.069 fg/mL. By using the proposed strategy to detect CFHR1 from
urine, it showed acceptable accuracy, which can effectively distinguish
between bladder cancer patients and healthy samples. This work contributes
to a novel, noninvasive, and accurate method for early clinical diagnosis
of bladder cancer.