am1c17912_si_001.pdf (935.37 kB)
Ultrasensitive Dual-Signal Detection of Telomerase and MiR-21 Based on Boolean Logic Operations
journal contribution
posted on 2021-10-19, 16:35 authored by Jiaju Shi, Meiqi Shen, Wenjie Zhao, Jinhua Liu, Zongjin Qu, Mengting Zhu, Zichao Chen, Pengfei Shi, Zhen Zhang, Shu-Sheng ZhangTelomerase
and micro-RNAs (miRNAs) are simultaneously upregulated
in a variety of tumor cells and have emerged as promising tumor markers.
However, sensitive detection of telomerase and miRNAs in situ remains
a great challenge due to their low expression levels. Here, we designed
a Boolean logic “AND” signal amplification strategy
based on functionalized ordered mesoporous nanoparticles (FOMNs) to
achieve ultrasensitive detection of telomerase and miR-21 in living
tumor cells. Briefly, the strategy uses telomerase as an input to
enable the release of DNA3-ROX-BHQ hairpins by making the wrapping
DNA1 form a DNA-a hairpin with the joint participation of dNTPs. Subsequently,
DNA2-Ag, DNA3-ROX-BHQ, and the second input miR-21 participated in
hybridization chain reaction to amplify fluorescence and Raman signals.
Experimental results showed the intensity of output dual signals relevant
to the expression levels of telomerase and miR-21. The Ag nanoparticles
(AgNPs) not only enhanced the fluorescence signals but also allowed
to obtain more sensitive Raman signals. Therefore, even if expression
of tumor markers is at a low level, the FOMN-based dual-signal logic
operation strategy can still achieve sensitive detection of telomerase
and miR-21 in situ. Furthermore, FOMNs can detect miR-21 expression
levels in a short time. Consequently, this strategy has a potential
clinical application value in detection of tumor markers and the assessment
of tumor treatment efficacy.
History
Usage metrics
Categories
Keywords
wrapping dna1 formhybridization chain reactiongreat challenge dueexperimental results showedboolean logic “tumor treatment efficacypromising tumor markersliving tumor cellsachieve ultrasensitive detectionlow expression levelssensitive raman signalsstrategy uses telomerasesecond input mir21 expression levelstumor markerstumor cellsexpression levelsraman signalsultrasensitive dualsensitive detectionlow levelfluorescence signalssimultaneously upregulatedsignal detectionshort timejoint participationdetect mirbased dualamplify fluorescencealso allowed21 participated21 based