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Ultrasensitive Detection of Amyloid‑β Using Cellular Prion Protein on the Highly Conductive Au Nanoparticles–Poly(3,4-ethylene dioxythiophene)–Poly(thiophene-3-acetic acid) Composite Electrode
journal contribution
posted on 2019-08-13, 20:29 authored by Jieling Qin, Misuk Cho, Youngkwan LeeA highly sensitive
electrochemical impedance sensor for amyloid
beta oligomer (AβO) was fabricated using a cellular prion protein
(PrPC) bioreceptor linked with poly(thiophene-3-acetic
acid) transducer. An additional thin layer of poly(3,4-ethylene dioxythiophene)
embedded with gold nanoparticles was employed to provide high electrical
conductivity and a large surface area. The sensing performace was
investigated in terms of sensitivity and detection range. The fabricated
sensor exhibited extremely low detection limit at a subfemtomolar
level with a wide detection range from 10–8 to 104 nM and its utility was established in mice infected with
Alzheimer’s disease (AD). The developed AβO sensor could
be utilized for early diagnosis of AD.