Ultrafast Ratiometric Detection of Aflatoxin B1 Based on Fluorescent β‑CD@Cu Nanoparticles and Pt2+ Ions
journal contributionposted on 2021-12-16, 14:37 authored by Min Li, Zhi-Juan Qian, Chi-Fang Peng, Xin-Lin Wei, Zhou-Ping Wang
Rapid detection of aflatoxin B1 (AFB1) is a very important task in food safety monitoring. However, it is still challenging to achieve highly sensitive detection without antibody or aptamer biomolecules. In this work, a rapid detection of aflatoxin B1 was achieved using a ratiometric fluorescence probe without antibody or aptamer for the first time. In the ratiometric fluorescence system, the fluorescence emission of AFB1 at 433 nm was significantly enhanced due to the β-cyclodextrin–AFB1 host–guest interaction and the complexation of AFB1 and Pt2+. Meanwhile, the inclusion of aflatoxin B1 also quenched the fluorescence emission of β-CD@Cu nanoparticles (NPs) at 650 nm based on inner filter effect mechanism. On the basis of the above effects, the ratiometric detection of aflatoxin B1 was achieved in the range of 0.03–10 ng/mL with a low detection limit of 0.012 ng/mL (3σ/s). In addition, the β-CD@Cu NPs based nanoprobe could achieve stable response within 1 min to AFB1. The above ratiometric detection also demonstrated excellent application potential in the rapid on-site detection of AFB1 in food due to the advantages of convenience, rapidness, and high accuracy.
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significantly enhanced duelow detection limitfood safety monitoring650 nm basedultrafast ratiometric detectionratiometric fluorescence systemaflatoxin b1 based2 + supions rapid detectionratiometric detectionfood dueaflatoxin b1site detectionrapid detectionfluorescence emission433 nmstill challengingimportant taskhigh accuracyfirst timecu nanoparticles012 ng