UPLC-Q-TOF/MS-Based Serum Metabolomics Reveals the Anti-Ischemic Stroke Mechanism of Nuciferine in MCAO Rats
journal contributionposted on 2020-12-15, 22:13 authored by Lanlan Wu, Chang Chen, Yongbiao Li, Cong Guo, Yuqing Fan, Dingrong Yu, Tinglan Zhang, Binyu Wen, Zhiyong Yan, An Liu
Nuciferine is an aporphine alkaloid monomer that is extracted from the leaves of the lotus species Nymphaea caerulea and Nelumbo nucifera Gaertn. Nuciferine was reported to treat cerebrovascular diseases. However, the potential mechanism of the neuroprotective effects of nuciferine at the metabolomics level is still not unclear. The present research used neurological score, infarct volume, cerebral water content, and ultraperformance liquid chromatography to quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS)-based serum metabolomics to elucidate the anti-ischemic stroke effect and mechanisms of nuciferine. The results showed that nuciferine significantly improved neurological deficit scores and ameliorated cerebral edema and infarction. Multivariate data analysis methods were used to examine the differences in serum endogenous metabolism between groups, and the biomarkers of nuciferine on ischemic stroke were identified. Approximately 19 metabolites and 7 metabolic pathways associated with nuciferine on treatment of stroke were found, which indicated that nuciferine exerted a positive therapeutic action on cerebral ischemic by regulating metabolism. These results provided some data support for the study of anti-stroke effect of nuciferine from the perspective of metabolomics.
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metabolomics levelanti-ischemic stroke effectMultivariate data analysis methodsischemic strokeMCAO Rats Nuciferinewater contentaporphine alkaloid monomer19 metabolitesdata supportnuciferineinfarct volumeUPLC-Q-TOFcerebrovascular diseasesquadrupole time-of-flight mass spec...deficit scoresanti-stroke effectneuroprotective effectsNelumbo nucifera Gaertnlotus species Nymphaea caeruleaAnti-Ischemic Stroke Mechanism