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Two-Dimensional Difference Gel Electrophoresis Analysis of Streptococcus uberis in Response to Mutagenesis-Inducing Ciprofloxacin Challenge

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journal contribution
posted on 02.01.2009, 00:00 authored by Marjo Poutanen, Emilia Varhimo, Nisse Kalkkinen, Antti Sukura, Pekka Varmanen, Kirsi Savijoki
In Streptococcus uberis, the fluoroquinolone antibiotic ciprofloxacin induces a mutagenic response that is distinct from the SOS paradigm. Two-dimensional differential gel electrophoresis was employed to investigate the effect of ciprofloxacin exposure on the proteome of S. uberis. Twenty-four protein spots exhibiting differential expression (p < 0.05) were identified as enzymes with potential role in oxidative stress, NADH generation and nucleotide biosynthesis. We suggest that these metabolic changes provide S. uberis means to stimulate mutagenesis and adaptation.