Turn-On, Fluorescent Nuclear Stains with Live Cell Compatibility
journal contributionposted on 15.03.2013, 00:00 by Demar R. G. Pitter, Jens Wigenius, Adrienne S. Brown, James D. Baker, Fredrik Westerlund, James N. Wilson
DNA-binding, green and yellow fluorescent probes with excellent brightness and high on/off ratios are reported. The probes are membrane permeable, live-cell compatible, and optimally matched to 405 nm and 514 nm laser lines, making them attractive alternatives to UV-excited and blue emissive Hoechst 33342 and DAPI nuclear stains. Their electronic structure was investigated by optical spectroscopy supported by TD-DFT calculations. DNA binding is accompanied by 27- to 75-fold emission enhancements, and linear dichroism demonstrates that one dye is a groove binder while the other intercalates into DNA.