ja0c07377_si_001.pdf (1.81 MB)
Toward Two-Photon Absorbing Dyes with Unusually Potentiated Nonlinear Fluorescence Response
Version 2 2020-08-24, 14:07
Version 1 2020-08-21, 15:33
journal contribution
posted on 2020-08-24, 14:07 authored by Carlos Benitez-Martin, Shiming Li, Antonio Dominguez-Alfaro, Francisco Najera, Ezequiel Pérez-Inestrosa, Uwe Pischel, Joakim AndréassonThe
combination of two two-photon-induced processes in a Förster
resonance energy transfer (FRET)-operated photochromic fluorene-dithienylethene
dyad lays the foundation for the observation of a quartic dependence
of the fluorescence signal on the excitation light intensity. While
this photophysical behavior is predicted for a four-photon absorbing
dye, the herein proposed approach opens the way to use two-photon
absorbing dyes, reaching the same performance. Hence, the spatial
resolution limit, being a critical parameter for applications in fluorescence
imaging or data storage with common two-photon absorbing dyes, is
dramatically improved.