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Torque-Induced Slip of the Rotary Motor F1-ATPase

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journal contribution
posted on 13.10.2010, 00:00 by Akilan Palanisami, Tetsuaki Okamoto
F1-ATPase plays an essential role in cellular metabolism by linking rotational motion to ATP hydrolysis/synthesis. We measure the torque profile of F1 in both ATP hydrolysis and synthesis directions using a novel magnetic nanorod assay. F1 is found to decouple ATP synthesis from rotary motion at a surprisingly low torque. This low-torque slip mechanism protects the enzyme from excessive load and may play a broader biological role by reducing the production of reactive oxygen species.