Uncontrollable
hemorrhage, especially uncompressible and massive
hemorrhage, is life threatening. To develop a kind of hemostatic material
that is biocompatible and has effective hemostatic performance, a
starch-polyethylene glycol sponge (St-PEG sponge) incorporated with
electrospraying microspheres made from carboxymethyl chitosan and
sodium alginate (CMC/SA eMPs) is employed to fabricate the topical
hemostatic agent for application. To load thrombin safely and effectively,
CMC/SA eMPs encapsulating thrombin compounds (eMPs@Thr) are further
incorporated with St-PEG sponge to obtain eMPs@Thr/sponge. The results
show that eMPs@Thr/sponge could obviously reduce blood loss and shorten
the hemostatis time compared with commercial hemostatic products Kuai
Kang. The construction of the eMPs@Thr/sponge could not only maintain
the expandable properties of the St-PEG sponge but also strengthen
the procoagulant activity. Therefore, this work provides a facile
approach for loading thrombin given the fact that thrombin suffers
from instability and risk of thrombus. It is predicted that eMPs@Thr/sponge
might hold great potential in uncompressible and massive hemorrhage
control.