Thioredoxin
Reductase-Mediated Reaction Evokes In Situ Surface
Polarization Effect on BiOIO3: Toward a New Sensing Strategy
for Cathodic Photoelectrochemistry
posted on 2024-02-09, 23:04authored byMengmeng Gu, Zhangcong Yu, Xiuming Wu, Yuanyuan Sun, Jiangwei Hu, Yuming Dong, Guang-Li Wang
We have witnessed the fast progress
of cathodic photoelectrochemistry
over the past decades, though its signal transduction tactic still
lacks diversity. Exploring new sensing strategies for cathodic photoelectrochemistry
is extremely demanding yet hugely challenging. This article puts forward
a unique idea to incorporate an enzymatic reaction-invoked surface
polarization effect (SPE) on the surface of BiOIO3 to implement
an innovative cathodic photoelectrochemical (PEC) bioanalysis. Specifically,
the thioredoxin reductase (TrxR)-mediated reaction produced the polar
glutathione (GSH), which spontaneously coordinated to the surface
of BiOIO3 and induced SPE by forming a polarized electric
field, resulting in improved electron (e–) and hole
(h+) pair separation efficiency and an enhanced photocurrent
output. Correlating this phenomenon with the detection of TrxR exhibited
a high performance in terms of sensitivity and selectivity, achieving
a linear range of 0.007–0.5 μM and a low detection limit
of 2.0 nM (S/N = 3). This study brings refreshing inspiration for
the cathodic PEC signal transduction tactic through enzyme-mediated in situ reaction to introduce SPE, which enriches the diversity
of available signaling molecules. Moreover, this study unveils the
potential of in situ generated SPE for extended and
futuristic applications.