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Thiol–Ene Alginate Hydrogels as Versatile Bioinks for Bioprinting
journal contribution
posted on 2018-06-25, 00:00 authored by Huey Wen Ooi, Carlos Mota, A. Tessa ten Cate, Andrea Calore, Lorenzo Moroni, Matthew B. BakerBioprinting
is a powerful technique that allows precise and controlled
3D deposition of biomaterials in a predesigned, customizable, and
reproducible manner. Cell-laden hydrogel (“bioink”)
bioprinting is especially advantageous for tissue engineering applications
as multiple cells and biomaterial compositions can be selectively
dispensed to create spatially well-defined architectures. Despite
this promise, few hydrogel systems are easily available and suitable
as bioinks, with even fewer systems allowing for molecular design
of mechanical and biological properties. In this study, we report
the development of a norbornene functionalized alginate system as
a cell-laden bioink for extrusion-based bioprinting, with a rapid
UV-induced thiol–ene cross-linking mechanism that avoids acrylate
kinetic chain formation. The mechanical and swelling properties of
the hydrogels are tunable by varying the concentration, length, and
structure of dithiol PEG cross-linkers and can be further modified
by postprinting secondary cross-linking with divalent ions such as
calcium. The low concentrations of alginate needed (<2 wt %), coupled
with their rapid in situ gelation, allow both the
maintenance of high cell viability and the ability to fabricate large
multilayer or multibioink constructs with identical bioprinting conditions.
The modularity of this bioink platform design enables not only the
rational design of materials properties but also the gel’s
biofunctionality (as shown via RGD attachment) for the expected tissue-engineering
application. This modularity enables the creation of multizonal and
multicellular constructs utilizing a chemically similar bioink platform.
Such tailorable bioink platforms will enable increased complexity
in 3D bioprinted constructs.
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tissue-engineering applicationbiomaterial compositionschain formationhydrogel systemscell-laden bioinkbioprinting conditionsdivalent ionstissue engineering applications3 D depositionmaterials propertiesnorbornene functionalized alginate system3 D bioprintedbioink platformbioink platform designSuch tailorable bioink platformscell viabilityRGD attachmentVersatile Bioinksextrusion-based bioprintingBioprinting Bioprintingdithiol PEG cross-linkers
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