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Thermal Detection of Cardiac Biomarkers Heart-Fatty Acid Binding Protein and ST2 Using a Molecularly Imprinted Nanoparticle-Based Multiplex Sensor Platform
journal contribution
posted on 2019-10-15, 13:36 authored by Robert
D. Crapnell, Francesco Canfarotta, Joanna Czulak, Rhiannon Johnson, Kai Betlem, Francesco Mecozzi, Michael P. Down, Kasper Eersels, Bart van Grinsven, Thomas J. Cleij, Richard Law, Craig E. Banks, Marloes PeetersThis
manuscript describes the production of molecularly imprinted
polymer nanoparticles (nanoMIPs) for the cardiac biomarkers heart-fatty
acid binding protein (H-FABP) and ST2 by solid-phase synthesis, and
their use as synthetic antibodies in a multiplexed sensing platform.
Analysis by surface plasmon resonance (SPR) shows that the affinity
of the nanoMIPs is similar to that of commercially available antibodies.
The particles are coated onto the surface of thermocouples and inserted
into 3D-printed flow cells of different multiplexed designs. We demonstrate
that it is possible to selectively detect both cardiac biomarkers
within the physiologically relevant range. Furthermore, the developed
sensor platform is the first example of a multiplex format of this
thermal analysis technique which enables simultaneous measurements
of two different compounds with minimal cross selectivity. The format
where three thermocouples are positioned in parallel exhibits the
highest sensitivity, which is explained by modeling the heat flow
distribution within the flow cell. This design is used in further
experiments and proof-of-application of the sensor platform is provided
by measuring spiked fetal bovine serum samples. Because of the high
selectivity, short measurement time, and low cost of this array format,
it provides an interesting alternative to traditional immunoassays.
The use of nanoMIPs enables a multimarker strategy, which has the
potential to contribute to sustainable healthcare by improving the
reliability of cardiac biomarker testing.
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heat flow distributionSPRsensor platformthermocoupleST 2selectivitymeasurementH-FABPCardiac Biomarkers Heart-Fattybiomarkers heart-fatty acid binding proteinantibodyMolecularly Imprinted Nanoparticle-Based Multiplex Sensor PlatformnanoMIPsurface plasmon resonance3 D-printed flow cellsmultiplexedanalysis
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