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Therapeutic Effectiveness of Gardenia jasminoides on Type 2 Diabetic Rats: Mass Spectrometry-Based Metabolomics Approach
journal contribution
posted on 2020-08-29, 17:15 authored by Lu Wang, Chen Yang, Fengrui Song, Zhiqiang Liu, Shu LiuGardenia jasminoides fruits, a kind
of traditional Chinese medicine (TCM), have shown prospects in the
prevention of diabetes and its complications. However, due to their
chemical diversity and multiple biological targets, the effective
mechanism remains largely unknown. In this study, mass spectrometry-based
metabolomics was applied to uncover the underlying therapeutic mechanism
of G. jasminoides on type 2 diabetes
(T2D) and its clinical complications. We established a T2D rat model
using high-fat diet and identified 45 urinary endogenous metabolites
as potential diagnostic biomarkers for T2D rats. After treatment with G. jasminoides, pathologic symptoms of T2D rats were
significantly improved and some of the diagnostic biomarkers were
significantly regulated. Bioinformatics analysis demonstrated that
these therapeutic biomarkers were involved in bile acid biosynthesis,
amino acid metabolism, vitamin B metabolism, taurine metabolism, etc.,
which indicated potential therapeutic mechanisms of G. jasminoides on T2D and its complications.