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The Akt C-Terminal Modulator Protein Is an Acyl-CoA Thioesterase of the Hotdog-Fold Family

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journal contribution
posted on 23.06.2009, 00:00 by Hong Zhao, Brian M. Martin, Marco Bisoffi, Debra Dunaway-Mariano
Herein, we report on an in vitro kinetic activity analysis that demonstrates that the protein known as the Akt C-terminal modulator protein is a broad-range, high-activity acyl-CoA thioesterase. In vitro tests of possible activity regulation by product inhibition or by Akt1 binding gave negative results. Truncation mutants confined the thioesterase activity to the C-terminal domain, consistent with our threading model. The N-terminal domain of unknown fold and function was found to contribute to solubility.