Tentative Identification of Phenolic Compounds in
Olive Pomace Extracts Using Liquid Chromatography–Tandem Mass
Spectrometry with a Quadrupole–Quadrupole-Time-of-Flight Mass
Detector
posted on 2012-11-21, 00:00authored byÁngela Peralbo-Molina, Feliciano Priego-Capote, María Dolores Luque de Castro
The reuse of agronomical residues is a pending goal for sustainable
agriculture. Particular residues in olive-oil-producing countries
are leaves, wastewater, and olive pomace. Olive leaves and wastewaters
have been previously characterized by isolation of the phenolic fraction.
However, olive pomace has not yet been qualitatively characterized
as a source of phenols. Olive pomace extracts were obtained using
superheated solvent extraction using 50:50 (v/v) water/ethanol as
a leaching mixture at 160 °C. The extracts were analyzed by liquid
chromatography coupled to tandem mass spectrometry using a quadrupole–quadrupole-time-of-flight
(QqTOF) hybrid mass analyzer (R = 25 000–45 000).
Qualitative analysis was supported upon measurement of accurate masses
for precursor and product ions as well as their isotopic distribution.
Identification was focused on the main families of phenolic compounds
present in extra virgin olive oil. The potential of this residue as
a rich source of phenols with antioxidant properties has been proven.