ac9b04474_si_001.pdf (1.61 MB)
Download fileTMTpro: Design, Synthesis, and Initial Evaluation of a Proline-Based Isobaric 16-Plex Tandem Mass Tag Reagent Set
journal contribution
posted on 03.12.2019, 18:34 authored by Andrew Thompson, Nikolai Wölmer, Sasa Koncarevic, Stefan Selzer, Gitte Böhm, Harald Legner, Peter Schmid, Stefan Kienle, Petra Penning, Claudia Höhle, Antje Berfelde, Roxana Martinez-Pinna, Vadim Farztdinov, Stephan Jung, Karsten Kuhn, Ian PikeThe design and synthesis of a proline-based reporter
isobaric Tandem
Mass Tag structure (TMTpro) is presented. An analysis is made of the
performance of the new TMTpro tags in comparison with the current
commercially available dimethylpiperidine-reporter-based TMT10/11
reagents. The new reporter structure provides a set of 16 tags for
use with resolution of 6.3 mDa mass differences in high resolution
mass spectrometers and a set of 9 reagents with 1 Da spacing between
reporter ions for single dalton analysis using 9 heavy nuclei per
tag. We show similar performance in terms of peptide identification
rates and quantification between the TMTpro 16-plex and TMT10/11-plex
reagents. We also demonstrate the suitability of the TMTpro reagents
for phosphopeptide analysis. The ability to pool 16 samples reduces
the overall amount of sample required for each channel, and we anticipate
that TMTpro reagents will be a useful enhancement for any protocol
that benefits from sample pooling and should reduce missing data.