sb8b00533_si_001.pdf (109.81 kB)
TASBE Flow Analytics: A Package for Calibrated Flow Cytometry Analysis
journal contribution
posted on 2019-05-03, 00:00 authored by Jacob Beal, Cassandra Overney, Aaron Adler, Fusun Yaman, Lisa Tiberio, Meher SamineniFlow cytometry is a powerful method
for high-throughput precision
measurement of cell fluorescence and size. Effective use of this tool
for quantification of synthetic biology devices and circuits, however,
generally requires careful application of complex multistage workflows
for calibration, filtering, and analysis with appropriate statistics.
The TASBE Flow Analytics package provides a free, open, and accessible
implementation of such workflows in a form designed for high-throughput
analysis of large synthetic biology data sets. Given a set of experimental
samples and controls, this package can process them to output calibrated
data, quantitative analyses and comparisons, automatically generated
figures, and detailed debugging and diagnostic reports in both human-readable
and machine-readable forms. TASBE Flow Analytics can be used through
a simple user-friendly interactive Excel interface, as a library supporting
Matlab, Octave, or Python interactive sessions, or as a component
integrated into automated workflows.