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Synthesis of O‑Sulfated Human Syndecan-1-like Glyco-polypeptides by Incorporating Peptide Ligation and O‑Sulfated Glycopeptide Cassette Strategies

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journal contribution
posted on 06.08.2020 by Tianlu Li, Weizhun Yang, Sherif Ramadan, Xuefei Huang
A successful synthesis of O-sulfated syndecan-1-like (Q23–E120) glyco-polypeptide was accomplished. The synthesis features the integration of an O-sulfated carbohydrate-bearing glycopeptide cassette with efficient protein ligation strategies, overcoming the acid lability of carbohydrate sulfates as a major hurdle in solid-phase peptide synthesis. Crucial to the synthesis is the microwave-assisted Ag­(I) ligation, which afforded the ligation product in improved overall yield. This O-sulfated syndecan-1 (Q23–E120) is the longest O-sulfated glyco-polypeptide prepared to date.

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