Synthesis of O‑Sulfated Human Syndecan-1-like Glyco-polypeptides by Incorporating Peptide Ligation and O‑Sulfated Glycopeptide Cassette Strategies
journal contributionposted on 06.08.2020 by Tianlu Li, Weizhun Yang, Sherif Ramadan, Xuefei Huang
Any type of content formally published in an academic journal, usually following a peer-review process.
A successful synthesis of O-sulfated syndecan-1-like (Q23–E120) glyco-polypeptide was accomplished. The synthesis features the integration of an O-sulfated carbohydrate-bearing glycopeptide cassette with efficient protein ligation strategies, overcoming the acid lability of carbohydrate sulfates as a major hurdle in solid-phase peptide synthesis. Crucial to the synthesis is the microwave-assisted Ag(I) ligation, which afforded the ligation product in improved overall yield. This O-sulfated syndecan-1 (Q23–E120) is the longest O-sulfated glyco-polypeptide prepared to date.