ol0c02243_si_001.pdf (5.55 MB)
Synthesis of O‑Sulfated Human Syndecan-1-like Glyco-polypeptides by Incorporating Peptide Ligation and O‑Sulfated Glycopeptide Cassette Strategies
journal contribution
posted on 2020-08-06, 22:22 authored by Tianlu Li, Weizhun Yang, Sherif Ramadan, Xuefei HuangA successful
synthesis of O-sulfated syndecan-1-like
(Q23–E120) glyco-polypeptide was accomplished. The synthesis
features the integration of an O-sulfated carbohydrate-bearing
glycopeptide cassette with efficient protein ligation strategies,
overcoming the acid lability of carbohydrate sulfates as a major hurdle
in solid-phase peptide synthesis. Crucial to the synthesis is the
microwave-assisted Ag(I) ligation, which afforded the ligation product
in improved overall yield. This O-sulfated syndecan-1
(Q23–E120) is the longest O-sulfated glyco-polypeptide
prepared to date.