am6b15919_si_001.pdf (1.37 MB)
Synthesis of a Cationic Supramolecular Block Copolymer with Covalent and Noncovalent Polymer Blocks for Gene Delivery
journal contribution
posted on 2017-02-24, 00:00 authored by Wumaier Yasen, Ruijiao Dong, Linzhu Zhou, Jieli Wu, Chengxi Cao, Aliya Aini, Xinyuan ZhuThe
design and fabrication of safe and highly efficient nonviral
vectors is the key scientific issue for the achievement of clinical
gene therapy. Supramolecular cationic polymers have unique structures
and specific functions compared to covalent cationic polymers, such
as low cytotoxicity, excellent biodegradability, and smart environmental
responsiveness, thereby showing great application prospect for gene
therapy. However, supramolecular gene vectors are facile to be degraded
under physiological conditions, leading to a significant reduction
of gene transfection efficiency. In order to achieve highly efficient
gene expression, it is necessary for supramolecular gene vectors being
provided with appropriate biostability to overcome various cell obstacles.
To this end, a novel cationic supramolecular block copolymer composed
of a conventional polymer and a noncovalent polymer was constructed
through robust β-cyclodextrin/ferrocene host–guest recognition.
The resultant supramolecular block copolymer perfectly combines the
advantages of both conventional polymers and supramolecular polymers
ranging from structures to functions. This supramolecular copolymer
not only has the ability to effectively condense pDNA for enhanced
cell uptake, but also releases pDNA inside cancer cells triggered
by H2O2, which can be utilized as a prospective
nonviral delivery vehicle for gene delivery. The block polymer exhibited
low cytotoxicity, good biostability, excellent biodegradability, and
intelligent responsiveness, ascribing to the dynamic/reversible nature
of noncovalent linkages. In vitro studies further illustrated that
the supramolecular block polymer exhibited greatly improved gene transfection
efficiency in cancer cells. This work offers an alternative platform
for the exploitation of smart nonviral vehicles for specific cancer
gene therapy in the future.
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Cationic Supramolecular Block Copolymercovalent cationic polymerscancer cellsnovel cationic supramolecular block copolymernonviral delivery vehiclesupramolecular block copolymerSupramolecular cationic polymerscancer gene therapyNoncovalent Polymer Blockssupramolecular block polymerH 2 O 2gene transfection efficiencygene therapysupramolecular gene vectors
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