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Synthesis of Water-Dispersible Molecularly Imprinted Electroactive Nanoparticles for the Sensitive and Selective Paracetamol Detection
journal contribution
posted on 2016-07-27, 00:00 authored by Jing Luo, Qiang Ma, Wei Wei, Ye Zhu, Ren Liu, Xiaoya LiuA novel kind of water-dispersible
molecularly imprinted electroactive
nanoparticles was prepared combining macromolecular self-assembly
with molecularly imprinting technique employing paracetamol (PCM)
as template molecule. An amphiphilic electroactive copolymer (P(NVC-EHA-AA),
PNEA) containing carbazole group was first synthesized through a one-pot
free radical copolymerization. The coassembly of the electroactive
copolymers with the template molecules (PCM) in aqueous solution generated
nanoparticles embedded with PCM, leading to the formation of molecularly
imprinted electroactive nanoparticles (MIENPs). A robust MIP film
was formed on the surface of electrode by electrodeposition of MIENPs
and subsequent electropolymerization of the carbazole units in MIENPs.
After the extraction of PCM molecules, a MIP sensor was successfully
constructed. It should be noted that electropolymerization of the
electroactive units in MIENPs creates cross-conjugated polymer network,
which not only locks the recognition sites but also significantly
accelerates the electron transfer and thus enhances the response signal
of the MIP sensor. These advantages endowed the MIP sensor with good
selectivity and high sensitivity for PCM detection. The MIP sensor
could recognize PCM from its possible interfering substances with
good selectivity. Under the optimal conditions, two linear ranges
from 1 μM to 0.1 mM and 0.1 to 10 mM with a detection limit
of 0.3 μM were obtained for PCM detection. The MIP sensor also
showed good stability and repeatability, which has been successfully
used to analyze PCM in tablets and human urine samples with satisfactory
results.