Synthesis and Screening of α‑Xylosides in Human Glioblastoma Cells
journal contributionposted on 14.12.2020, 20:33 by Mausam Kalita, Javier Villanueva-Meyer, Yuki Ohkawa, Chakrapani Kalyanaraman, Katharine Chen, Esraa Mohamed, Matthew F. L. Parker, Matthew P. Jacobson, Joanna J. Phillips, Michael J. Evans, David M. Wilson
Glycosaminoglycans (GAGs) such as heparan sulfate and chondroitin sulfate decorate all mammalian cell surfaces. These mucopolysaccharides act as coreceptors for extracellular ligands, regulating cell signaling, growth, proliferation, and adhesion. In glioblastoma, the most common type of primary malignant brain tumor, dysregulated GAG biosynthesis results in altered chain length, sulfation patterns, and the ratio of contributing monosaccharides. These events contribute to the loss of normal cellular function, initiating and sustaining malignant growth. Disruption of the aberrant cell surface GAGs with small molecule inhibitors of GAG biosynthetic enzymes is a potential therapeutic approach to blocking the rogue signaling and proliferation in glioma, including glioblastoma. Previously, 4-azido-xylose-α-UDP sugar inhibited both xylosyltransferase (XYLT-1) and β-1,4-galactosyltransferase-7 (β-GALT-7)the first and second enzymes of GAG biosynthesiswhen microinjected into a cell. In another study, 4-deoxy-4-fluoro-β-xylosides inhibited β-GALT-7 at 1 mM concentration in vitro. In this work, we seek to solve the enduring problem of drug delivery to human glioma cells at low concentrations. We developed a library of hydrophobic, presumed prodrugs 4-deoxy-4-fluoro-2,3-dibenzoyl-(α- or β-) xylosides and their corresponding hydrophilic inhibitors of XYLT-1 and β-GALT-7 enzymes. The prodrugs were designed to be activatable by carboxylesterase enzymes overexpressed in glioblastoma. Using a colorimetric MTT assay in human glioblastoma cell lines, we identified a prodrug–drug pair (4-nitrophenyl-α-xylosides) as lead drug candidates. The candidates arrest U251 cell growth at an IC50 = 380 nM (prodrug), 122 μM (drug), and U87 cells at IC50 = 10.57 μM (prodrug). Molecular docking studies were consistent with preferred binding of the α- versus β-nitro xyloside conformer to XYLT-1 and β-GALT-7 enzymes.
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β- nitro xyloside conformerHuman Glioblastoma Cells Glycosamin...glioblastoma cell lines1 mM concentrationcarboxylesterase enzymes overexpressedcolorimetric MTT assaycell surface GAGsU 87 cellsMolecular docking studiesβ- GALT -710.57 μ MGAG biosynthetic enzymesβ- GALT -7 enzymesdysregulated GAG biosynthesis resultscandidates arrest U 251 cell growthXYLTIC 50prodrugs 4- deoxy -4-fluoro4- azido-xylose -α-UDP sugar4- deoxy -4-fluoro