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Synthesis and RNA Binding Selectivity of Oligonucleotides Modified with Five-Atom Thioacetamido Nucleic Acid Backbone Structures

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posted on 05.07.2007, 00:00 by Khirud Gogoi, Anita D. Gunjal, Usha D. Phalgune, Vaijayanti A. Kumar
Convenient chemical synthesis and incorporation of dithymidine and thymidine−cytidine dimer blocks connected with a five-atom amide linker N3‘−CO−CH2−S−CH2 into oligonucleotides (ONs) are reported. The UV−Tm experiments for binding affinities of these mixed backbone ONs with complementary DNA and RNA sequences revealed important results such as significantly higher RNA-binding selectivity as compared with complementary DNA. NMR studies of the dimer blocks suggested a marginal increase in the N-type sugar conformations over that of the native DNA.

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