Synthesis and Evaluation of a New Fluorescent Transglycosylase Substrate: Lipid II-Based Molecule Possessing a Dansyl-C20 Polyprenyl Moiety
journal contribution
posted on 2010-04-02, 00:00 authored by Chen-Yu Liu, Chih-Wei Guo, Yi-Fan Chang, Jen-Tsung Wang, Hao-Wei Shih, Yu-Fang Hsu, Chia-Wei Chen, Shao-Kang Chen, Yen-Chih Wang, Ting-Jen R. Cheng, Che Ma, Chi-Huey Wong, Jim-Min Fang, Wei-Chieh ChengThe preparation of a novel fluorescent lipid II-based substrate for transglycosylases (TGases) is described. This substrate has characteristic structural features including a shorter lipid chain, a fluorophore tag at the end of the lipid chain rather than on the peptide chain, and no labeling with a radioactive atom. This fluorescent substrate is readily utilized in TGase activity assays to characterize TGases and also to evaluate the activities of TGase inhibitors.
