Synthesis and Application
of Two-Photon Active Fluorescent
Rhodol Dyes for Antibody Conjugation and In Vitro Cell Imaging

Szepesi Kovács, Dénes; Chiovini, Balázs; Müller, Dalma; Tóth, Estilla Zsófia; Fülöp, Anna; Ábrányi-Balogh, Péter; Wittner, Lucia; Várady, György; Farkas, Ödön; Turczel, Gábor; Katona, Gergely; Győrffy, Balázs; Keserű, György Miklós; Mucsi, Zoltán; Rózsa, Balázs J.; Kovács, Ervin

doi:10.1021/acsomega.3c01796.s001

Synthesis and Application of Two-Photon Active Fluorescent Rhodol Dyes for Antibody Conjugation and In Vitro Cell Imaging

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posted on 2023-06-14, 09:06 authored by Dénes Szepesi Kovács, Balázs Chiovini, Dalma Müller, Estilla Zsófia Tóth, Anna Fülöp, Péter Ábrányi-Balogh, Lucia Wittner, György Várady, Ödön Farkas, Gábor Turczel, Gergely Katona, Balázs Győrffy, György Miklós Keserű, Zoltán Mucsi, Balázs J. Rózsa, Ervin Kovács

A novel family of julolidine-containing fluorescent rhodols equipped with a wide variety of substituents was synthesized in a versatile two-step process. The prepared compounds were fully characterized and exhibited excellent fluorescence properties for microscopy imaging. The best candidate was conjugated to the therapeutic antibody trastuzumab through a copper-free strain-promoted azide-alkyne click reaction. The rhodol-labeled antibody was successfully applied for in vitro confocal and two-photon microscopy imaging of Her2+ cells.

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alkyne click reaction therapeutic antibody trastuzumab photon microscopy imaging microscopy imaging labeled antibody cell imaging antibody conjugation wide variety vitro successfully applied step process promoted azide prepared compounds novel family fully characterized free strain best candidate

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Synthesis and Application of Two-Photon Active Fluorescent Rhodol Dyes for Antibody Conjugation and In Vitro Cell Imaging

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