Synthesis, Tumor Specificity, and Photosensitizing Efficacy of Erlotinib-Conjugated Chlorins and Bacteriochlorins: Identification of a Highly Effective Candidate for Photodynamic Therapy of Cancer
journal contributionposted on 05.01.2021, 20:16 by Ravindra R. Cheruku, Joseph Cacaccio, Farukh A. Durrani, Walter A. Tabaczynski, Ramona Watson, Kevin Siters, Joseph R. Missert, Erin C. Tracy, Mykhaylo Dukh, Khurshid Guru, Richard C. Koya, Pawel Kalinski, Heinz Baumann, Ravindra K. Pandey
Erlotinib was covalently linked to 3-(1′-hexyloxy)ethyl-3-devinylpyropheophorbide-a (HPPH) and structurally related chlorins and bacteriochlorins at different positions of the tetrapyrrole ring. The functional consequence of each modification was determined by quantifying the uptake and subcellular deposition of the erlotinib conjugates, cellular response to therapeutic light treatment in tissue cultures, and in eliminating of corresponding tumors grown as a xenograft in SCID mice. The experimental human cancer models the established cell lines UMUC3 (bladder), FaDu (hypopharynx), and primary cultures of head and neck tumor cells. The effectiveness of the compounds was compared to that of HPPH. Furthermore, specific functional contribution of the carboxylic acid side group at position 172 and the chiral methyl group at 3(1′) to the overall activity of the chimeric compounds was assessed. Among the conjugates investigated, the PS 10 was identified as the most effective candidate for achieving tumor cell-specific accumulation and yielding improved long-term tumor control.
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PS 10tissue culturesSCID micecancer modelsHPPHcell lines UMUC 3tumor controlposition 17 2Effective CandidateTumor Specificitytetrapyrrole ringchimeric compoundserlotinib conjugatesCancer Erlotinibcarboxylic acid side groupchiral methyl groupErlotinib-Conjugated Chlorinstumor cell-specific accumulationPhotodynamic Therapyneck tumor cellslight treatmentsubcellular depositionPhotosensitizing Efficacy