Synthesis, Chaperoning, and Metabolism of Proteins Are Regulated by NT-3/TrkC Signaling in the Medulloblastoma Cell Line DAOY
journal contributionposted on 02.05.2008, 00:00 by Mariella Gruber-Olipitz, Thomas Ströbel, Wei-Qiang Chen, Michael A. Grotzer, Franz Quehenberger, Irene Slavc, Gert Lubec
The human medulloblastoma cell line DAOY was transfected with Tropomyosin receptor kinase (TrkC), a marker for good prognostic outcome. Following TrkC-activation by its ligand neurotrophin-3, protein extracts from DAOY cells were run on 2DE with subsequent MALDI-TOF-TOF analysis and quantification in order to detect downstream effectors. Protein levels of translational, splicing, processing, chaperone, protein handling, and metabolism machineries were shown to depend on neurotrophin-3-induced TrkC activation probably representing pharmacological targets.
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translationalProtein levelsquantificationmarkerTrkCextractMetabolismanalysisDAOY cellsprognostic outcomeMedulloblastoma Cell Line DAOYTheSignaling2 DEtransfectedTropomyosin receptor kinaseNTSynthesiactivationProteinsneurotrophinligandeffectormedulloblastoma cell line DAOYprotein handlingchaperonemetabolism machineriesChaperoningRegulated