Suspension Array with Shape-Coded Silica Nanotubes for Multiplexed Immunoassays

A suspension array for multiplexed immunoassays has been developed using shape-coded silica nanotubes (SNTs) as coding materials. Fabricated by multistep anodization template synthesis, each shape-coded SNT has several segments with different reflectance values depending on their diameters and wall thicknesses. Therefore, the code of each SNT can be “read-out” under a conventional optical microscope. The suspension array with shape-coded SNTs has shown high stability and dispersibility in aqueous buffer media and high detection sensitivity. The SNTs have not shown any visible degradation while submerged in aqueous solution for 7 months, the tubular structure and silanol groups on the inner and outer surfaces allow SNTs to disperse evenly in buffer solution, and the detection limit of an IgG protein is about 6 pM with 1.5 × 10⁶ SNTs per mL. We have demonstrated the high selectivity of the SNTs suspension array for the detection of multianalytes in the multiplexed immunoassay experiments.