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Supramolecular Approach to Enzyme Sensing on Paper Discs Using Lanthanide Photoluminescence

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journal contribution
posted on 21.06.2016, 00:00 by Tumpa Gorai, Uday Maitra
A paper based photoluminescent biosensor has been developed, with green Tb-luminescence as the output, for the rapid detection of β-glucosidase and lipase, both in purified form and in biological/natural fluids. A Tb Cholate hydrogel doped with specially designed, specific enzyme substrates (″pro″-sensitizers) was integrated on filter paper discs.The addition of small volumes (∼5 μL) of enzyme solutions on these discs led to enhanced green emission (UV lamp, λex 365 nm), allowing qualitative detection of the enzymeswhen needed, the intensity enhancement can be quantified using image processing software, or for multiple samples using a commercial plate reader. This simple technique allowed the detection of β-glucosidase in almond extract and lipase in blood serum. Easy identification of the inhibition of β-glucosidase was also demonstrated. The paper based sensor is inexpensive, user-friendly and needs low volumes of biological sample for analysis. This strategy has the potential to be useful for possible clinical applications.