Supramolecular Approach to Enzyme Sensing on Paper Discs Using Lanthanide Photoluminescence
journal contributionposted on 21.06.2016, 00:00 by Tumpa Gorai, Uday Maitra
A paper based photoluminescent biosensor has been developed, with green Tb-luminescence as the output, for the rapid detection of β-glucosidase and lipase, both in purified form and in biological/natural fluids. A Tb Cholate hydrogel doped with specially designed, specific enzyme substrates (″pro″-sensitizers) was integrated on filter paper discs.The addition of small volumes (∼5 μL) of enzyme solutions on these discs led to enhanced green emission (UV lamp, λex 365 nm), allowing qualitative detection of the enzymeswhen needed, the intensity enhancement can be quantified using image processing software, or for multiple samples using a commercial plate reader. This simple technique allowed the detection of β-glucosidase in almond extract and lipase in blood serum. Easy identification of the inhibition of β-glucosidase was also demonstrated. The paper based sensor is inexpensive, user-friendly and needs low volumes of biological sample for analysis. This strategy has the potential to be useful for possible clinical applications.