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Superwettable Electrochemical Biosensor toward Detection of Cancer Biomarkers
journal contribution
posted on 2018-01-08, 00:00 authored by Tailin Xu, Yongchao Song, Wei Gao, Tingting Wu, Li-Ping Xu, Xueji Zhang, Shutao WangBioinspired
superwettable micropatterns that combine two extreme
states of superhydrophobicity and superhydrophilicity with the ability
to enrich and absorb microdroplets are suitable for versatile and
robust sensing applications. Here we introduce a superwettable microchip
that integrates superhydrophobic–superhydrophilic micropatterns
and a nanodendritic electrochemical biosensor toward the detection
of prostate cancer biomarkers. On the superwettable microchip, the
superhydrophobic area could confine the microdroplets in superhydrophilic
microwells; such behavior is extremely helpful for reducing the amount
of analytical solution. In contrast, superhydrophilic microwells exhibit
a high adhesive force toward microdroplets, and the nanodendritic
structures can improve probe-binding capacity and response signals,
thus greatly enhancing the sensitivity. Sensitive and selective detection
of prostate cancer biomarkers including miRNA-375, miRNA-141, and
prostate-specific antigen on a single microchip is also achieved.
Such a superwettable microchip with high sensitivity, low sample volume,
and upside-down detection capability in a single microdroplet shows
great potential to fabricate portable devices toward complex biosensing
applications.
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sensitivitynanodendritic electrochemical biosensorapplicationsuperwettable microchipmicrodropletsuperhydrophilic microwells exhibitCancer Biomarkers Bioinspired superwettable micropatternsprostate cancer biomarkersSuperwettable Electrochemical BiosensormiRNAsuperhydrophobicupside-down detection capability
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