Structure and Sodium Channel Activity of an Excitatory I1-Superfamily Conotoxin†,‡
journal contributionposted on 04.09.2007, 00:00 by Olga Buczek, Daxiu Wei, Jeffrey J. Babon, Xiaodong Yang, Brian Fiedler, Ping Chen, Doju Yoshikami, Baldomero M. Olivera, Grzegorz Bulaj, Raymond S. Norton
Conotoxin ι-RXIA, from the fish-hunting species Conus radiatus, is a member of the recently characterized I1-superfamily, which contains eight cysteine residues arranged in a -C-C-CC-CC-C-C- pattern. ι-RXIA (formerly designated r11a) is one of three characterized I1 peptides in which the third last residue is posttranslationally isomerized to the d configuration. Naturally occurring ι-RXIA with d-Phe44 is significantly more active as an excitotoxin than the l-Phe analogue both in vitro and in vivo. We have determined the solution structures of both forms by NMR spectroscopy, the first for an I1-superfamily member. The disulfide connectivities were determined from structure calculations and confirmed chemically as 5−19, 12−22, 18−27, and 21−38, suggesting that ι-RXIA has an ICK structural motif with one additional disulfide (21−38). Indeed, apart from the first few residues, the structure is well defined up to around residue 35 and does adopt an ICK structure. The C-terminal region, including Phe44, is disordered. Comparison of the d-Phe44 and l-Phe44 forms indicates that the switch from one enantiomer to the other has very little effect on the structure, even though it is clearly important for receptor interaction based on activity data. Finally, we identify the target of ι-RXIA as a voltage-gated sodium channel; ι-RXIA is an agonist, shifting the voltage dependence of activation of mouse NaV1.6 expressed in Xenopus oocytes to more hyperpolarized potentials. Thus, there is a convergence of structure and function in ι-RXIA, as its disulfide pairing and structure resemble those of funnel web spider toxins that also target sodium channels.
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voltage dependenceSodium Channel Activityresidue 35solution structuresstructure calculationsreceptor interactiondisulfide pairinghyperpolarized potentialsr 11amouse NaV 1.6disulfide connectivities1 peptidesXenopus oocytesICK structureposttranslationally isomerizedactivity dataNMR spectroscopycysteine residuesRXIAweb spider toxinstarget sodium channels