posted on 2020-06-01, 09:29authored byHuifang Chen, Kai Wang, Hang Xiao, Zhuoyan Hu, Lei Zhao
The objective of
this study was to extract and purify proteins
from the pulp tissues of litchi and evaluate their structure and pro-inflammatory
activity. The results showed that a highly pure litchi protein was
identified as the litchi thaumatin-like protein (LcTLP) by nano LC–MS/MS
and verified by sequencing of the LcTLP gene. The molecular weight
was 24 kDa, and the main secondary structure was a β sheet (33.00
± 2.86%). Small-angle X-ray scattering results showed that LcTLP
was a spherical particle (diameter of approximately 140 to 165 nm)
with a close internal and rough surface in solution. The assay of
pro-inflammatory activity in vitro revealed that
the expression of inducible nitric oxide synthase and cyclooxygenase-2
genes reached 9.71 ± 0.64 and 7.05 ± 1.00 after 200 μg/mL
LcTLP stimulation, which were 7.05-fold and 9.61-fold that of the
blank control, respectively. LcTLP promoted the gene expression and
production of pro-inflammatory cytokines, including tumor necrosis
factor-α and interleukin-1β, and it also enhanced the
expression of p65, which is a key component of nuclear factor-κ
B signaling pathways. Additionally, the levels of anti-inflammatory
cytokines interleukin-10 and transforming growth factor-β1 increased
after LcTLP stimulation.