Stepwise Filtering of the Internal Layers of Dendrimers by Transverse-Relaxation-Edited NMR

The characteristic distribution of transverse relaxation times (T₂) within dendrimers (shorter values at the core than the periphery) can be exploited in T₂-edited 1D and 2D NMR experiments for the stepwise filtering of internal nuclei according to their topology within the dendritic structure. The resulting filtered spectra, which can be conceived as corresponding to virtual hollow dendrimers, benefit from reduced signal overlap, thus facilitating signal assignment and characterization. The generality of the method as a powerful tool in structural and end-group analysis has been confirmed with various dendritic families and nuclei (¹H, ¹³C, ³¹P).