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Stable Tris(2,2′-bipyridine)ruthenium(III) for Chemiluminescence Detection

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journal contribution
posted on 01.07.2011, 00:00 authored by Geoffrey P. McDermott, Philip Jones, Neil W. Barnett, David N. Donaldson, Paul S. Francis
Two exceedingly stable [Ru(bipy)3]3+ reagents were prepared by dissolving either [Ru(bipy)3](ClO4)2 in acetonitrile (containing 0.05 M HClO4) or [Ru(bipy)3]Cl2·6H2O in 95:5 glacial acetic acid–acetic anhydride (containing 0.05 M H2SO4) followed by oxidation with PbO2. These conveniently prepared solutions provide highly reproducible chemiluminescence detection over long periods of analysis, avoiding the need for recalibration or preparation of fresh reagent solutions and without the complications associated with online chemical or electrochemical oxidations. The reagent prepared in acetonitrile produced much greater signal intensities with a range of analytes and was deemed most suitable for high-performance liquid chromatography (HPLC) with postcolumn chemiluminescence detection.