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Stabilization of Folded Peptide and Protein Structures via Distance Matching with a Long, Rigid Cross-Linker

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journal contribution
posted on 21.11.2007, 00:00 by Fuzhong Zhang, Oleg Sadovski, Steven J. Xin, G. Andrew Woolley
Intramolecular cross-linking is predicted to stabilize the folded state of peptides and proteins most effectively if the cross-linker provides a rigid link that is well-matched in end-to-end distance with attachment sites in the peptide or protein. We describe a thiol-reactive sulfonated alkyne-based cross-linker that is demonstrably more effective than more flexible counterparts. Exceptional stabilization of helical structure in short peptides is obtained.