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Download fileSpatially Controlled Photochemical Peptide and Polymer Conjugation on Biosurfaces
journal contribution
posted on 2013-12-09, 00:00 authored by Thomas Tischer, Tanja K. Claus, Michael Bruns, Vanessa Trouillet, Katharina Linkert, Cesar Rodriguez-Emmenegger, Anja S. Goldmann, Sébastien Perrier, Hans G. Börner, Christopher Barner-KowollikAn
efficient phototriggered Diels–Alder conjugation is utilized
to graft in an effective and straightforward approach poly(trifluoro
ethyl methacrylate) (Mn = 3700 Da, Đ = 1.27) and a model peptide (GIGKFLHS) onto thin
hyaluronan films and cellulose surfaces. The surfaces were functionalized
with an o-quinodimethane moiety – capable
of releasing a caged diene – via carbodiimide mediated coupling.
The o-quinodimethane group is employed as a photoactive
linker to tether predefined peptide/polymer strands in a spatially
controlled manner onto the biosurface by photoenol ligation. An in-depth
characterization employing XPS, ToF-SIMS, SPR, ellipsometry, and AFM
was conducted to evidence the effectiveness of the presented approach.
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Keywords
Polymer Conjugationfunctionalizedellipsometryphototriggereddienemannermodel peptidehyaluronan filmspredefinedmoietyethylcellulose surfacesphotoenol ligationSPRconjugationtethergraftapproachcarbodiimideDieleffectivenessMnSpatially Controlled Photochemical Peptidepolyphotoactive linker3700 DaAFMstrandXPSevidencecharacterizationGIGKFLHSbiosurfaceBiosurfacesAnmethacrylate