Spatially Controlled Photochemical Peptide and Polymer Conjugation on Biosurfaces
journal contributionposted on 09.12.2013, 00:00 by Thomas Tischer, Tanja K. Claus, Michael Bruns, Vanessa Trouillet, Katharina Linkert, Cesar Rodriguez-Emmenegger, Anja S. Goldmann, Sébastien Perrier, Hans G. Börner, Christopher Barner-Kowollik
An efficient phototriggered Diels–Alder conjugation is utilized to graft in an effective and straightforward approach poly(trifluoro ethyl methacrylate) (Mn = 3700 Da, Đ = 1.27) and a model peptide (GIGKFLHS) onto thin hyaluronan films and cellulose surfaces. The surfaces were functionalized with an o-quinodimethane moiety – capable of releasing a caged diene – via carbodiimide mediated coupling. The o-quinodimethane group is employed as a photoactive linker to tether predefined peptide/polymer strands in a spatially controlled manner onto the biosurface by photoenol ligation. An in-depth characterization employing XPS, ToF-SIMS, SPR, ellipsometry, and AFM was conducted to evidence the effectiveness of the presented approach.
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Polymer Conjugationfunctionalizedellipsometryphototriggereddienemannermodel peptidehyaluronan filmspredefinedmoietyethylcellulose surfacesphotoenol ligationSPRconjugationtethergraftapproachcarbodiimideDieleffectivenessMnSpatially Controlled Photochemical Peptidepolyphotoactive linker3700 DaAFMstrandXPSevidencecharacterizationGIGKFLHSbiosurfaceBiosurfacesAnmethacrylate