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Sizing Nanomatter in Biological Fluids by Fluorescence Single Particle Tracking

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journal contribution
posted on 10.11.2010, 00:00 by Kevin Braeckmans, Kevin Buyens, Wim Bouquet, Chris Vervaet, Philippe Joye, Filip De Vos, Laurent Plawinski, Loïc Doeuvre, Eduardo Angles-Cano, Niek N. Sanders, Jo Demeester, Stefaan C. De Smedt
Accurate sizing of nanoparticles in biological media is important for drug delivery and biomedical imaging applications since size directly influences the nanoparticle processing and nanotoxicity in vivo. Using fluorescence single particle tracking we have succeeded for the first time in following the aggregation of drug delivery nanoparticles in real time in undiluted whole blood. We demonstrate that, by using a suitable surface functionalization, nanoparticle aggregation in the blood circulation is prevented to a large extent.