Size-Controlled Synthesis of Modifiable Glycine-Terminated Au Nanoclusters as a Platform for Further Functionalization

An improved and simple synthetic method for producing stable narrow-sized glycine-cystamine (Gly-CSA)-functionalized Au nanoclusters (NCs) from protected Fmoc-glycine-cystamine (Fmoc-Gly-CSA)-functionalized Au NCs is demonstrated in this study. The NC size and size distribution can be controlled directly as a function of reducing agent concentration with the formation of smaller NC core diameters at higher concentrations of NaBH₄. Furthermore, when using 0.30 M NaBH₄, three UV–vis absorption peaks at 690, 440, and 390 nm were seen, which are consistent with the formation of Fmoc-Gly-CSA-functionalized Au₂₅L₁₈ NCs. After deprotection of the Gly-CSA-functionalized Au NCs, the reactivity of the primary amine groups was investigated. Methyl acrylate-glycine-cystamine (MA-Gly-CSA)-functionalized Au NCs with terminal acetyl groups were formed via the Michael addition reaction of terminal amine groups with methyl acrylate. This reaction resulted in the formation of ester-terminated Au NCs including atom-precise MA-Gly-CSA Au₂₅(SR)₁₈ NCs. The functionalization of the ligand was confirmed by ¹H NMR and UV–vis spectra, and TEM images of MA-Gly-CSA- and Gly-CSA-functionalized Au NCs showed that the size of the NCs remained unchanged after the reaction. With controllable NC size and facile functionalization of the Gly-CSA-functionalized Au NCs, these clusters have promising potential as scaffolds for biomedical applications.