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Single Site Discrimination of Cytosine, 5‑Methylcytosine, and 5‑Hydroxymethylcytosine in Target DNA Using Anthracene-Tagged Fluorescent Probes

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journal contribution
posted on 18.11.2015, 00:00 by Jean-Louis H. A. Duprey, Gemma A. Bullen, Zheng-yun Zhao, Dario M. Bassani, Anna F. A. Peacock, John Wilkie, James H. R. Tucker
The ability to discriminate between epigenetic variants in DNA is a necessary tool if we are to increase our understanding of the roles that they play in various biological processes and medical conditions. Herein, it is demonstrated how a simple two-step fluorescent probe assay can be used to differentiate all three major epigenetic variants of cytosine at a single locus site in a target strand of DNA.