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Single-Phase Dy2O3:Tb3+ Nanocrystals as Dual-Modal Contrast Agent for High Field Magnetic Resonance and Optical Imaging

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journal contribution
posted on 10.05.2011, 00:00 by Gautom Kumar Das, Yan Zhang, Loyola D’Silva, Parasuraman Padmanabhan, Boon Chin Heng, Joachim Say Chye Loo, Subramanian Tamil Selvan, Kishore K. Bhakoo, Timothy Thatt Yang Tan
Ultrasmall terbium ions (Tb3+) doped dysprosium oxide (Dy2O3) nanocrystals (NCs) of ∼3 nm diameter have been synthesized and applied as a single-phase dual modality contrast agent for high field magnetic resonance imaging (MRI) and optical imaging. The NCs show excellent positive contrast at 7.0 T MRI in phantom and in vivo imaging in a mouse breast tumor model (MCF-7 cells) injected with NCs. In vitro confocal fluorescence microscopy images show the staining of cultured human bronchial epithelial cells (BEAS-2B) with green fluorescent NCs. In vitro cytotoxicity studies in BEAS-2B and L929 cells indicate that the bifunctional NCs have no appreciable toxicity at a concentration of up to 1000 μg/mL. These results suggest the potential application of these NCs as dual modality contrast agents in a combined high field MRI and fluorescence imaging.